1.浙江大學 生物系統工程與食品科學學院;2.浙江大學 化學系
1.College of Biosystems Engineering and Food Science,Zhejiang University;2.Department of Chemistry , Zhejiang University
通過比較不同油茶副產品提取物的體外酪氨酸酶活性抑制作用,篩選出最佳抑制效果的油茶花提取物,并以曲酸作為陽性對照,采用體外培養的人A375黑素瘤細胞模型和斑馬魚胚胎模型探究其對體內外酪氨酸酶活性和黑色素合成的影響。結果表明,油茶花、油茶葉、油茶蒲和油茶粕提取物均表現出較好的酪氨酸酶活性抑制效果,其中油茶花效果最佳,其抑制IC50為0.49 mg/mL。與曲酸相比,油茶花提取物表現出極顯著的胞內酪氨酸酶活性抑制作用和黑色素合成抑制作用(p<0.01),作用濃度為160 μg/mL時,胞內酪氨酸酶活性的抑制率達到34.30%,相對黑素含量減少了26.41%。當油茶花提取物暴露濃度為100 μg/mL時,其對斑馬魚體內酪氨酸酶抑制率達到32.47%,相對黑素含量降低至64.39%,作用效果與曲酸相當。油茶花提取物在體內外均表現出了較好的酪氨酸酶抑制效果,對細胞和斑馬魚黑色素生成具有顯著抑制作用,且安全性高,是一種潛在的美白功能成分,在日用化妝品領域具有較高的應用前景。
By comparing the different Camellia oleifera extracts from by-products of the tyrosinase activity inhibition in vitro, and select the best inhibiting effect of Camellia oleifera flowers extract, and using the kojic acid as the positive control, using human A375 melanoma cells in vitro culture model and zebrafish embryo model to explore its influence of tyrosinase activity and melanin synthesis in vivo and in vitro. The results showed that Camellia oleifera flowers extract, Camellia oleifera leaves extract, Camellia oleifera shell extract and Camellia oleifera meal extract showed good inhibitory effects on tyrosinase activity, and Camellia oleifera flowers extract had the best inhibitory effect with an IC50 of 0.49 mg/mL. Compared with kojic acid, Camellia oleifera flowers extract showed significant inhibition of intracellular tyrosinase activity and melanin synthesis (P <0.01). When the concentration was 160 μg/mL, the inhibition rate of intracellular tyrosinase activity reached 34.30%, and the relative melanin content decreased by 26.41%. When the exposure concentration of Camellia oleifera flowers extract was 100μg/mL, the inhibition rate of tyrosinase in zebrafish reached 32.47%, and the relative melanin content was reduced to 64.39%. The effect was similar to kojic acid. Camellia oleifera flowers extract showed good tyrosinase inhibition in vitro and in vivo, and significantly inhibited melanin production in cells and zebrafish, with high safety. It is a potential whitening functional component, and has a high application prospect in the field of daily cosmetics.